Effect of Bacteria Inoculums on Compressive Strength

Pooja  Bandekar; Sandhay  Basavaraj; Prakash Mallappa Munnoli; Jyoti  Gupta; Geeta  Shetteppanavar; Sudisha  Jogayya

doi:10.15415/jotitt.2020.82008

Authors

Pooja Bandekar SDM College of Engineering and Technology, Dharwad, Karnataka-580002, India
Sandhay Basavaraj SDM College of Engineering and Technology, Dharwad, Karnataka-580002, India
Prakash Mallappa Munnoli SDM College of Engineering and Technology, Dharwad, Karnataka-580002, India
Jyoti Gupta SDM College of Engineering and Technology, Dharwad, Karnataka-580002, India
Geeta Shetteppanavar SDM College of Engineering and Technology, Dharwad, Karnataka-580002, India
Sudisha Jogayya Department of Biotechnology and Microbiology, KUD Dharwad, Karnataka-580003, India

DOI:

https://doi.org/10.15415/jotitt.2020.82008

Keywords:

Calcite precipitation, Bio-deposition, Compressive strength, Bacillus Subtilis, Bacillus Pumilis, Pseudomonas Fluorescence

Abstract

The use of bio-concrete is increasing in the present day context and researchers are working on strength and durability characteristics of concrete using bacteria species which have shown calcite precipitation. Three different species of bacteria namely P. Fluorescence, B. Pumilis and B. Subliis that have calcite precipitation properties have been investigated in this study. The investigations were carried first on cement mortar (CM) cubes using these three bacteria species suspension of 20%; 40% and 60% having colony forming units P. Fluorescence (10⁸ CFU/ml), B. Pumilis (10⁶ CFU/ml) and B. Subtilis (10⁸ CFU/ml) respectively. The 40% suspension in all the three cases has shown increased compressive strength as compared to 20% and 60%. The compressive strength measured showed increase (CS) of 18%; 12% for P. Fluorescence; B. Subtilis and decrease of 35% with B. Pumilis respectively. B. Subtilis with optimized 40% suspension having CFU 10x10⁸/ml showed 4.32% ; 5.56%; and 3.81% increase in CS of CC cubes with 3 days; 7 days and 28 days respectively and 5.92% overall increase in CS of CC cubes as compared to the 3 days CS of control cube.
ABBREVIATIONS
SDW: Sterile Distilled Water; SHC: Self-Healing Concrete; PCR: Polymerase Chain Reaction; BC: Bacterial Concrete; CP: Calcite precipitation; CS: Compressive Strength; CC: Cement Concrete; CM: Cement Mortar; MTCC: Microbial Type Culture Collection; CFU: Colony Forming Unit/ml

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